You will use the 5th day on your lobby notice. See the below model form taken directly from the Reg:
C-18--Notice at locations where employees accept consumer deposits
(case-by-case holds)
FUNDS AVAILABILITY POLICY
Our general policy is to allow you to withdraw funds deposited in your account on the (number) business day after the day we receive your deposit. Funds from electronic direct deposits will be available on the day we receive the deposit. In some cases, we may delay your ability to withdraw funds beyond the (number) business day. Then, the funds will generally be available by the fifth business day after the day of deposit.
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In the end, it's all just a bunch of paper....