KK:
If you use computer-generated signature cards, program the software to only print out the number of signature lines as you have applicants. You may also have the ability to print a custom phrase when the card is generated, such as "THIS ACCOUNT HAS TWO (2) AUTHORIZED SIGNERS".
If the new accounts representative types information on to a pre-printed card, use a red, felt-tip, water-soluble pen to line out the unused lines. Any attempt to add a signature will require the obliteration of the red line by using a liquid ("Liquid Paper") or paper-based (label) product. Either means will still show the alteration -- as a runny, pink mess or a pink label.