Most likely not.
Reviewing the check though, is there a spray or endorsement on the back of the check before it was presented to you? Some of the better scanners will 'spray' the check, therefor indicating to the accepting bank that this item may have already been electronically deposited.
Depending on how your conversation goes with the account owner as well, you may consider not banking this customer any further. Either they are being sneaky and fraudulent, or they have a poor system of internal controls in their place of business that would allow this item to be physically deposited as well.
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I don't repeat gossip, so listen closely...